pe conjugated anti mouse grm3  (Bioss)

Journal: Current Neuropharmacology

Article Title: mGlu3 Metabotropic Glutamate Receptors as a Target for the Treatment of Absence Epilepsy: Preclinical and Human Genetics Data

doi: 10.2174/1570159X20666220509160511

Figure Lengend Snippet: mGlu3 receptor protein levels in the thalamus and somatosensory cortex of WAG/Rij and control rats. Representative immunoblot of mGlu3 receptor levels in the thalamus and somatosensory cortex of pre-symptomatic (25/27 days of age) and symptomatic (6/7 months of age) WAG/Rij rats and control Wistar rats. Densitometric analysis is the mean ± SEM (n=4-6). * p <0.05 vs. control rats (Student’s t test). A’ Thalamus : 25/27 days of age t=3,594, df=9; 95 percent two-tailed confidence interval for difference of means:0,0473 to 0,208; Two-tailed p =0,00580; 6/7 months of age t=6,912, df=10; 95 percent two-tailed confidence interval for difference of means:0,0765 to 0,149; Two-tailed p =0,0000413. A” Somatosensory cortex : 25/27 days of age t=3,283, df=7; 95 percent two-tailed confidence interval for difference of means:0,0362 to 0,222 ; Two-tailed p =0,0134; 6/7 months of age t=1,424, df=10; 95 percent two-tailed confidence interval for difference of means:-0,0570 to 0,259; Two-tailed p =0,185;

Article Snippet: Western blot analysis was performed as described before [ , ] using rabbit polyclonal antibodies for mGlu3 (AGC-012, Alomone Labs, 1:1000), GAT-1 (AB1570W, Millipore, 1:1000), GLAST (PA5-80012, ThermoFisher Scientific, 1:1000), GLT-1 (PA5-17099 ThermoFisher Scientific, 1:1000) or mouse monoclonal for GAPDH (sc-47724, Santa Cruz Biotechnology, 1:1000).

Techniques: Control, Western Blot, Two Tailed Test

Journal: Current Neuropharmacology

Article Title: mGlu3 Metabotropic Glutamate Receptors as a Target for the Treatment of Absence Epilepsy: Preclinical and Human Genetics Data

doi: 10.2174/1570159X20666220509160511

Figure Lengend Snippet: mGlu3 receptor mRNA levels in the thalamus of WAG/Rij and control rats. mGlu3 receptor mRNA in the thalamus and somatosensory cortex of WAG/Rij and control rats of 25/27 days or 6-7 months of age. Values are means±S.E.M. (n=4). * p <0.05 vs. control rats (Student’s t test). B’ Thalamus : 25/27 days of age t=-3,950 df=6; 95 percent two-tailed confidence interval for difference of means:-47951,329 to -11268,309; Two-tailed p =0,00754; 6/7 months of age t=1,816, df=6; 95 percent two-tailed confidence interval for difference of means:-16289,729 to 110127,774; Two-tailed p = 0,119. B” Somatosensory cortex : 25/27 days of age t=-2,110, df=6; 95 percent two-tailed confidence interval for difference of means:-170784,081 to 12626,584 ; Two-tailed p =0,0794 ; 6/7 months of age t=0,753, df=6; 95 percent two-tailed confidence interval for difference of means:-134142,946 to 253420,350; Two-tailed p =0,480.

Article Snippet: Western blot analysis was performed as described before [ , ] using rabbit polyclonal antibodies for mGlu3 (AGC-012, Alomone Labs, 1:1000), GAT-1 (AB1570W, Millipore, 1:1000), GLAST (PA5-80012, ThermoFisher Scientific, 1:1000), GLT-1 (PA5-17099 ThermoFisher Scientific, 1:1000) or mouse monoclonal for GAPDH (sc-47724, Santa Cruz Biotechnology, 1:1000).

Techniques: Control, Two Tailed Test

Journal: Current Neuropharmacology

Article Title: mGlu3 Metabotropic Glutamate Receptors as a Target for the Treatment of Absence Epilepsy: Preclinical and Human Genetics Data

doi: 10.2174/1570159X20666220509160511

Figure Lengend Snippet: Blunted mGlu3 receptor-mediated inhibition of cAMP formation in the thalamus and somatosensory cortex of WAG/Rij rats. cAMP formation in slices prepared from the thalamus and somatosensory cortex of WAG/Rij and control rats of 25/27 days and 6/7 months of age. Values are means ± S.E.M. (n=4-6). Statistical significance vs. Vehicle (*) or vs. Forskolin (#) (Two-Way ANOVA + Fisher's LSD test). C’ Twenty-five/twenty-seven days old rats, Somatosensory cortex: genotype, p =0,048, F (1,18) =4,517; treatment, p <0,001, F (2,18) =38,12; genotype x treatment, p =0,015, F (2,18) =5,304; Thalamus: genotype, p =0,292, F (1,18) =1,180; treatment, p <0,001, F (2,18) =14,746; genotype x treatment, p =0,936, F (2,18) =0,0660. C” Six/seven months old rats, Somatosensory cortex: genotype, p =0,008, F (1,29) =8,072; treatment, p <0,001, F (2,29) =41,18; genotype x treatment, p =0,041, F (2,29) =3,588; Thalamus: genotype, p =0,127, F (1,29) =2,463; treatment, p <0,001, F (2,29) =15,669; genotype x treatment, p =0,643, F (2,29) =0,449).

Article Snippet: Western blot analysis was performed as described before [ , ] using rabbit polyclonal antibodies for mGlu3 (AGC-012, Alomone Labs, 1:1000), GAT-1 (AB1570W, Millipore, 1:1000), GLAST (PA5-80012, ThermoFisher Scientific, 1:1000), GLT-1 (PA5-17099 ThermoFisher Scientific, 1:1000) or mouse monoclonal for GAPDH (sc-47724, Santa Cruz Biotechnology, 1:1000).

Techniques: Inhibition, Control

Journal: Current Neuropharmacology

Article Title: mGlu3 Metabotropic Glutamate Receptors as a Target for the Treatment of Absence Epilepsy: Preclinical and Human Genetics Data

doi: 10.2174/1570159X20666220509160511

Figure Lengend Snippet: Effect of mGlu3 receptor activation on mGlu5-mediated PI hydrolysis in cortical and thalamic slices of control and WAG/Rij rats. Polyphosphoinositide hydrolysis in slices prepared from the thalamus and somatosensory cortex of WAG/Rij and control rats of 25/27 days and 6-7 months of age. Values are means ± S.E.M. (n=4-7). Statistical significance vs. Vehicle (*) or vs. DHPG (#) (Two - Way ANOVA + Fisher's LSD test). D’ Twenty-five/twenty-seven days old rats, Somatosensory cortex : genotype, p =0,015, F (1,18) =7,302; treatment, p <0,001, F (2,18) =41,379; genotype x treatment, p =0,024, F (2,18) =4,620; Thalamus : genotype, p <0,001, F (1,27) =21,490; treatment, p <0,001, F (2,27) =48,54; genotype x treatment, p =0,011, F (2,27) =5,343. D” Six/seven months old rats, Somatosensory cortex : genotype, p<0,001 , F (1,44) =16,51; treatment, p =0,819, F (4,44) =0,385; Thalamus : genotype, p =0,007, F (1,36) =8,099; treatment, p =0,003, F (3,36) =5,754; genotype x treatment, p =0,373, F (3,36) =1,072).

Article Snippet: Western blot analysis was performed as described before [ , ] using rabbit polyclonal antibodies for mGlu3 (AGC-012, Alomone Labs, 1:1000), GAT-1 (AB1570W, Millipore, 1:1000), GLAST (PA5-80012, ThermoFisher Scientific, 1:1000), GLT-1 (PA5-17099 ThermoFisher Scientific, 1:1000) or mouse monoclonal for GAPDH (sc-47724, Santa Cruz Biotechnology, 1:1000).

Techniques: Activation Assay, Control

Journal: Frontiers in Molecular Neuroscience

Article Title: Sensory Input-Dependent Changes in Glutamatergic Neurotransmission- Related Genes and Proteins in the Adult Rat Trigeminal Ganglion

doi: 10.3389/fnmol.2016.00132

Figure Lengend Snippet: Lateral differences in protein levels in the TG. (A) Representative immunoblots showing protein levels of GLUR2, NMDAR2B, mGLUR3, and PICK1 in left (L) and right (R) sides of the TG in the same animal from Control, Enriched, and Trimmed groups. β3-tubulin was used as loading control. (B) Variation in protein expression level is given taking the right side as 100%. Left over right side differences in protein levels of GLUR2, NMDAR2B, mGLUR3, and PICK1 in the TG. Error bars indicate SEM. ∗ p < 0.05, ∗∗ p < 0.01 compared with relative control groups; student’s paired two-tailed t -test.

Article Snippet: Then, membranes were incubated with the following antibodies: rabbit polyclonal anti-mGLUR3 (1:500, Abcam, Cambridge, MA, USA), mouse monoclonal anti-GLUR2 (1:500, NeuroMab, Davis, CA, USA), mouse monoclonal anti-NMDAR2B (1:500, NeuroMab, Davis, CA, USA), mouse monoclonal anti-PICK1 (1 μg/ml, Abcam, Cambridge, MA, USA), rabbit polyclonal anti- PSD95 (1:1000, Cell Signaling Technologies, Beverly, MA, USA), and mouse monoclonal anti-β3-tubulin (1:10000, Calbiochem, La Jolla, CA, USA).

Techniques: Western Blot, Expressing, Two Tailed Test

Journal: Frontiers in Molecular Neuroscience

Article Title: Sensory Input-Dependent Changes in Glutamatergic Neurotransmission- Related Genes and Proteins in the Adult Rat Trigeminal Ganglion

doi: 10.3389/fnmol.2016.00132

Figure Lengend Snippet: Side-by-side comparison between groups of GLUR2 (A) , NMDAR2B (B) , mGLUR3 (C) , and PICK1 (D) protein levels in the TG. Error bars indicate SEM. ∗ p < 0.05, ∗∗ p < 0.01; student’s unpaired two-tailed t -test.

Article Snippet: Then, membranes were incubated with the following antibodies: rabbit polyclonal anti-mGLUR3 (1:500, Abcam, Cambridge, MA, USA), mouse monoclonal anti-GLUR2 (1:500, NeuroMab, Davis, CA, USA), mouse monoclonal anti-NMDAR2B (1:500, NeuroMab, Davis, CA, USA), mouse monoclonal anti-PICK1 (1 μg/ml, Abcam, Cambridge, MA, USA), rabbit polyclonal anti- PSD95 (1:1000, Cell Signaling Technologies, Beverly, MA, USA), and mouse monoclonal anti-β3-tubulin (1:10000, Calbiochem, La Jolla, CA, USA).

Techniques: Two Tailed Test